QTL seq for lobedness degree in tomato

This study aim was to identify new tomato QTLs for lobedness degree by the QTL-seq approach. For this purpose, we develop two segregatin populations by intraspecific crosses between tomato cultivars with the same allelic composition at main shape genes and contrasting values for lobedness degree. We selected the individuals with more extremes values for interest trait, we conformed DNA-bulks, we sequenced the samples, and mapped the variants between them for identified genomic regions underlying the lobedness degree. These regions were also validated by molecular marker analysis.