the role of neutrophil-produced collagen in skin injury

to assess the role of neutrophil-produced collagen in skin injury. 3 mouse groups (WT, Mrp8-Cre;TGFbR2-floxed and Mrp8-Cre;iDTR) were subjected to an ear injury by piercing. A limited area around the wound was dissected (6-10 mg tissue) and subjected to ECM extraction and TMT proteomics analysis. From each animal, an equivalent amount of skin from the other (healthy) ear was also excised and used as an internal control. 4 biological replicates per condition (WT, A and B) with 2 samples per animal (injured and healthy control) were distributed in three independent TMT 10-plex experiments. Samples from the same animal were kept together in the same TMT experiment. In each TMT experiment one of the channels was reserved for a common internal standard (I.S.) made by pooling the 24 peptide samples.