rG4-seq for P. aeruginosa and E. coli

Escherichia coli is the most widely studied strains, which has irreplaceable position in medicine and biology research. Pseudomonas aeruginosa, an opportunistic human pathogen, tends to cause potentially lethal acute or chronic infections in patients with cystic fibrosis (CF), immunocompromised individuals and burn victims. However, it is little know about the effect of the special secondary structure rG4 (G-quadruplex) in the mRNA on virulence regulation. Here, we aim to reveal the new and important post-transcriptional regulatory roles of rG4 in bacterial pathogenicity and metabolic pathways. Four samples for P. aeruginosa, 4 samples for E.coli, 150 bp single-ended RNA-Seq libraries from bacterial cell extracts after performing RTS reaction in different buffers (Li and K rich buffer). Each of the 2 conditions has 2 libraries from independent biological replicates.