Structural statistics of the DEAF-1 MYND domain.

Statistics are given for the 20 lowest energy structures after water refinement out of 100 calculated. 1Distance restraints were derived from NOE peak intensities using CYANA [31], and then introduced as unambiguous distances in CNS. No distance restraint was violated by more than 0.5 Å. 2Torsion angles were predicted using TALOS+ [30]. No dihedral angle restraint was violated by more than 5°. 3RDC restraints were incorporated using a harmonic potential. Force constants of 0.2, 0.1, 0.3, and 0.6 kcal mol−1 Hz−2 for HHN, NC′, HNC′ and Hα Cα respectively, were used to reflect the estimated error in the measurement. 4Ramachandran plot statistics were obtained using PROCHECK [64] for residues 502–541.