IL-17A and IL-22 synergistically stimulated human epithelial cells inducing IL-8.

(A) IL-8 production at 24 hours by AGS cells in response to IL-22 and IL-17A as measured by ELISA. p-value is based on ANOVA test with Dunnett’s correction for multiple comparisons ***p<0.001. (B) 8 hour stimulation of AGS cells or (C) AZ-521 cells with an IL-22/IL-17A cocktail induces upregulation of antimicrobials as measured by real-time rtPCR. Expression is shown as relative units and is relative to RNA from untreated cells. Data shown as ± SEM and are representative of 3 independent experiments.