Whole-genome sequencing data of plant edited by Mb2Cas12a

We established an efficient genome editing system in cotton mediated by CRISPR/Mb2Cas12a. First, we designed three vectors using various expression cassettes to verify their applicability in cotton.We have successfully obtained gossypol-free plants and leaf-bleaching plants separately, proving that all three vectors can perform efficient editing in cotton. Furthermore, we have revealed that Mb2Cas12a exhibited high precision through whole-genome sequencing, and no off-target effects were detected at the whole-genome level. In addition, effective editing at the relaxed TTV PAM site was successfully detected. Finally, a multiplex genome editing system was constructed by using CRISPR/Mb2Cas12a system to simultaneously target seven sites in a single crRNA array with TTTV PAMs.