Long-read nanopore sequencing of a pancreatic cancer cell culture derived from a KPC mouse.

Long-read sequencing libraries were prepared using the Oxford Nanopore LSK109 kit (ONT, Oxford, UK). 400 ng of library was loaded on a promethION flowcell and run for 72h on a promethION beta sequencer (ONT, Oxford, UK). Base-calling was performed on the promethION compute unit's GPU using guppy 3.2.8 basecaller. The resulting FASTQ file was mapped to the reference genome GRCm38.p6 using Minimap2 (option map-ont). Read coverage was extracted using bam-read count (minimum mapping quality 60, minimum base quality 5).