Anther Dehiscence

To estimate the pollen presentation schedule in each population, we tracked anther dehiscence in ten male-phase flowers on each day of data collection. Prior to first anther dehiscence, we arbitrarily chose ten fresh male-phase flowers and marked them with dental floss and a label. Every 30 minutes, we recorded the temperature and then returned to these flowers and recorded the number of locules dehisced (two locules per anther for a total of ten locules). We concluded tracking each flower when all ten locules had dehisced or, if the ten locules did not dehisce completely, when insect activity ceased.