Oncogenes hijack the stress response machinery in T cell acute lymphoblastic leukemia [GRO-Seq]

Oncogenes NOTCH1 and MYC directly regulate HSF1 and other critical proteins of the stress-response pathway in T-ALL. This GRO-Seq experiment demonstrates that release from NOTCH1 inhibition results in upregulation of HSF1 and other key HSPs. Nascent RNA was extracted from 10 million CUTLL1 cells using Trizol extraction (Invitrogen) according to the manufacturer’s protocol following the global run-on (GRO) reaction and BrUTP labeling. Nascent BrUTP-containing RNA was purified using BrdU beads (Santa Cruz) which was then used for library preparation. Libraries were sequenced on the Illumina HiSeq 2000 using 50bp single-read method. The experiment was performed at stready state (DMSO) and various time points post release from NOTCH1 signaling inhibition (γ-secretase inhibitor)