Prenatal LPS infection impacting on neonatal mice lung at birth

Pregnant wild type C57BL/6J mice were randomized at 15.5 dpc or 18.5 dpc to receive intraperitoneal (i.p.) injection of saline, LPS 50 µg/kg (LPS 50) or LPS 100 µg/kg (LPS 100). At birth, newborn pups were euthanized and 2 lobes of the right lung were removed and frozen in liquid nitrogen for RNA extraction. A total amount of 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by sequencing.