NIK-Driven IL-23 Production by Myeloid Cells is a Key Factor in the Development of Autoimmune Inflammation [scRNA-seq]

We have employed a single cell sequencing approach with BD Rhapsody to study the differences within myeloid cells in the draining lymph nodes four days after MOG immunisation between NIKfl/fl control mice and NIK^CX3CR1 mice Overall design: Following MOG immunisation cells were isolated from the inguinal lymph nodes at 5dpi. MACS sorting for CD90 and CD19 positive cells was used to remove T and B cells. enriched myeloid cells were analysed with scRNA-seq