The OEGMA-based hydrogel is active against all species of the ESKAPE pathogens, Streptococcus mutans and the pathogenic yeast Candida albicans

Wound dressings serve as a crucial first barrier against microbial contamination, particularly in severe wounds requiring rapid antimicrobial protection. Building on our previously established hydrogel system based on copolymers of oligo(ethylene glycol) methyl ether methacrylate (OEGMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) crosslinked during quaternization with 1,6-dibromohexane, we extend the biological evaluation of this contact-active material to a broader spectrum of clinically relevant pathogens. The hydrogel was assessed against the complete multidrug resistant ESKAPE group, the Gram-positive bacterium Staphylococcus mutans, and the opportunistic yeast Candida albicans. Pronounced contact-mediated antimicrobial activity was observed against all tested organisms, resulting in a significant reduction of microbial viability. Importantly, the material retained excellent biocompatibility, showing no adverse effects on mammalian skin fibroblasts or human blood cells. The combination of broad-spectrum antimicrobial efficacy, biological compatibility, and a robust yet simple fabrication approach highlights the potential of this hydrogel as a next-generation wound dressing and a promising first-line option for emergency wound care of critical injuries.