Characterization of NgAgo cleavage sites
收藏NIAID Data Ecosystem2026-04-29 收录
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资源简介：
NgAgo and mutants were incubated with plasmid DNA and 5'P guide sequences targeting the plasmid. Nicked/cleaved products were amplified via primer extension, which terminates at the nick site, before being used to generate dsDNA that was ligated into pGEM-T-Easy. The library of ligated products were then sequenced. Raw reads from products generated via primers hybridizing on the 5' and 3' end of the target are deposited here.
创建时间：
2021-07-21
