Supporting data for "Development of RNA Proximity Labeling Approaches Mediated by Fluorogenic Photosensitizers"

This dataset contains four subfiles, including 1) Organic synthesis of fluorogenic photosensitizers. Detailed synthetic routes of a series of malachite green derivatives and Hoechst-conjugated dibromofluorescien are recorded with the purification processes. 2) In vitro photophysical characterization of the synthetic compounds and the complex forming by binding to protein and DNA, including the UV-Vis absorption spectra, fluorescence spectra, and ROS detecting assays; 3) bacteria-related experiments which detailly record plasmid construction, amplification, protein expression, and purification; 4) mammalian cell-related experiments, including the microscope imaging of the synthetic compounds in live cells and visualization of the in situ labeling, analysis of protein and RNA isolated from labeled cells via western blot, in-gel fluorescence, dot blot, etc..

本数据集包含四个子文件，具体包括：1)一类荧光光敏剂的有机合成过程，详细记录了一系列孔雀石绿衍生物和Hoechst偶联的二溴荧光素的合成路线及纯化过程；2)对合成化合物及其与蛋白质和DNA结合形成的复合物的体外光物理表征，包括紫外-可见吸收光谱、荧光光谱以及活性氧检测实验；3)细菌相关实验，详细记录了质粒构建、扩增、蛋白表达及纯化过程；4)哺乳动物细胞相关实验，包括活细胞中合成化合物的显微镜成像、原位标记可视化，以及通过西方印迹、凝胶内荧光、点印迹等方法对标记细胞中分离的蛋白和RNA进行分析。