Real-time quantitative PCR analysis of colon tissues of DSS-induced colitis mice.

Inflammatory bowel disease (IBD) is a multifactorial disease involving defective immune responses against invasive microbiota. A Rab32-dependent pathway that eliminates intracellular bacteria in dendritic cells (DCs) was recently reported. In this study, we examined whether Rab32 in DCs participates in the pathogenesis of IBD WT and Rab32 dendritic cell specific knock out mice were administrated with 2.5% DSS for 9 days to induce colitis. On day 9, colon tissues were harvest for qPCR analysis. 3 mice in each group.