Isolation of human monoclonal antibodies from 4CMenB vaccinees reveals PorB and LOS as the main OMV components inducing cross-strain protection [OMV MenB]

In this study, we describe the development and use of an ad hoc protein microarray to characterize the meningococccal cross-binding of HumAbs isolated from 4CMenB vaccinated subjects and induced by the OMV component of the vaccine A panel of meningococcall Outer Membrane Vesicles (OMV) were printed randomly in replicates on a not-commercial protein microarray. The species-specific N.meningitidis OMV array was printed with different OMV in 20% glycerol. Controls consisted of 8 serial 2-fold dilutions of human IgG.