autosomal dominant polycystic kidney disease

Autosomaldominantpolycystickidneydisease(ADPKD) isacommonandcomplexhereditary nephropathy. Although most patients develop symptoms after adults, theybegin to bud in their fetal period. However, the underlying molecular mechanism andexact cause ofADPKD remain elusive. Therefore, based on the expression profile data ofperipheral blood from 3 patients with ADPKD and 3 normal controls sequenced byHuman Clariom D Array Chip, the core regulatory mechanism was explored with thecomprehensive circRNAs-miRNAs-mRNAs molecular target network analysis. In viewoftheinteraction mechanismof ceRNAs, weperformedcircRNAs-miRNAstargeting andtarget gene prediction for ADPKD-associated miRNAs, and identified interactionrelationship between 29 differential miRNAs and both 17 differential circRNAs and 1665differential mRNAs. These target genes are significantly involved in cell-matrix adhesionanddeposition,organmorphogenesis,inflammationandotherrelatedbiologicalprocessesand cellular components such as phagocytic vesicles, cytoplasmic vesicles, and renalcyst-associated biological signal pathways (eg, Hippo signaling pathway). Then, based onthe transcription and post-transcriptional regulation of circRNAs-target and RNA-relatedinteractions, significant regulators of the circRNAs-miRNAs-mRNAs molecular targetnetwork were identified as core regulators, including 5 circRNAs, 22 miRNAs, with GOand KEGG pathways Enrichment results demonstrating that critical circRNAssignificantly enriched the biological progress associated with organ fibrosis, renal injury,and cystic kidney disease. The key circRNAs compete with miRNAs as ceRNAs toachieve the regulation of miRNAs target gene expression levels to affect theADPKD-related genes and ultimately regulate its pathogenesis. Overall, our workdeciphers a network of circRNAs-miRNAs-mRNAs molecular targets for ADPKDhelping to reveal the core regulatory mechanisms of this disease and to increase ourunderstandingofitsunderlyingmolecularmechanisms.