16S rRNA Amplicon Sequencing of Human Cervicovaginal Lavage

The study utilized a cross sectional design, enrolling women with HIV or at risk for HIV infection, and it was conducted at the AIDS Clinical Research Unit at the University of Miami. The study was undertaken in cooperation with the Miami Women and HIV Interagency Study (WIHS) and the Miami Center for AIDS Research (CFAR). Eligibility criteria for participation were women 18 to 45 years of age and being sexually active in the 3 months previous to study enrollment. Pregnant women or women using contraceptive medications or intrauterine devices were excluded.