Global gene expression profiled with RNA-Seq in CYH33-resistant ESCC cells as well as their parental cells

To investigate the mechanism mediating resistance to CYH33 in ESCC cells, global gene expression was profiled with RNA-Seq in CYH33-resistant cells as well as their parental cells. Gene enrichment analysis revealed that RAS signaling pathway, PI3K-Akt signaling pathway and MAPK signaling pathway were highly activated in most CYH33-resistant cells. To analyze the difference of gene expression profiles between CYH33-resistant cells and their matched parental cells upon CYH33 treatment, cells were treated with CYH33 at 1 µM for 24 hours and RNA-seq was performed. Gene Set Enrichment Analysis was then carried out. Overall design: Total RNA profiles of 4 established CYH33-resistant ESCC cells and their parental cells treated with 1 µM CYH33 or Vehicle for 24 h