Partial epithelial-to-mesenchymal transition (EMT) and T cell-mediated inflammation in hypertensive and diabetic nephropathy

We comparatively evaluated transcriptomes from renal biopsies obtained from patients with T2DN or HN, main causes of CKD, and control renal tissues (n=6 per group). RNA was extracted from formalin-fixed and paraffin-embedded kidney samples and processed for RNA sequencing. Principal component analysis effectively separated diseased and control tissues. Gene and protein expression profiles revealed that EMT-related markers were upregulated in both diseases. Moreover, activation of CD4 and CD lymphocytes, as well as upregulation of the complement system were also observed in both T2DN and HN. These data, suggesting partially common pathogenetic mechanisms, were largely confirmed by IHC analysis. Formalin-fixed and paraffin-embedded kidney biopsies from adult patients were selected from the Norwegian Kidney Biopsy Registry. Specimens from patients with HN, T2DN and normal-appearing control renal biopsies (Ctrl) (n=6 for each group) were assessed. Total RNA was extracted from 10 μm whole kidney sections and processed for RNA sequencing, while immunohistochemistry (IHC) was performed on 3-μm tissue sections. *** Submitter declares that the raw data is being deposited in the European Genome-phenome Archive (EGA) due to patient privacy concerns.***