Microbial data and processing data of surface water in Xiantao constructed wetland

surface water samples directly put the sampler under the surface of water for 0.1-0.3 meters. DNA extraction were firstly collected into sterile Polyethylene plastic bottle(volume is at least 1L) using a sterilizing sampling bottle. The collected water samples are then stored in a box filled with sufficient ice to keep the sample temperature below 4°C during transit. When we arrived in the laboratory, we used microporous membrane (0.22 m) to filter water samples, filtered pore size filter membrane, stored in -80°C refrigerator, DNA was extracted with Water DNA Kit kit from OMEGA company, The extracted DNA was amplified using a specific set of bar-code 515F and 909R. Purification was recovered by Beijing Kang for century general DNA purification recovery Kit, and finally sent to the Chinese Academy of Sciences, Chengdu Institute of biology sequenced by using an Illumina Miseq platform DNA. In addition, Determination and analysis of water physical and chemical indexes: surface water (CODCr) was determined by potassium dichromate titration,TN by alkaline potassium persulfate digestion UV spectrophotometry spectrophotometric method, TP using potassium persulfate digestion ammonium molybdate spectrophotometric method, NH4+-N using Nessler's reagent colorimetric method.

水表面样品采集时，采样器直接置于水面以下0.1-0.3米处。DNA提取过程首先将样品收集于至少容积为1升的灭菌聚乙烯塑料瓶中，使用灭菌采样瓶进行。收集到的水样随后被置于装有充足冰块的箱内，以保持样品在运输过程中的温度低于4°C。抵达实验室后，我们采用0.22微米的微孔膜对水样进行过滤，存储于-80°C的冰箱中。使用OMEGA公司提供的Water DNA Kit提取DNA，通过特定的条形码515F和909R进行DNA扩增。纯化过程通过北京康为世纪通用DNA纯化回收试剂盒完成，最终将样品发送至中国科学院成都生物研究所，利用Illumina Miseq平台进行DNA测序。此外，对水体的物理化学指标进行测定和分析：表面水体中的化学需氧量（CODCr）通过重铬酸钾滴定法测定，总氮（TN）通过碱性过硫酸钾消解紫外分光光度法，总磷（TP）采用过硫酸钾消解钼酸铵分光光度法，氨氮（NH4+-N）则使用纳氏试剂比色法进行测定。