The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (ATAC-seq II)

Primary human fetal foreskin fibroblasts (HFFF) were infected with wild-type simplex virus 1 (HSV-1) strain F and null-ICP22 mutant at a multiplicity of infection (MOI) of 10. ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013 Overall design: HFFFs were infected with HSV-1 strain F or ICP22-null mutant for 8 or 12 hours. PAA was used at 350 µg/mL during the course of infection. ATAC-seq samples were prepared at the indicated times of infection. Two independent biological replicates were analysed.