Crebl2 regulates cell metabolism in muscle and liver cells (C2C12 RNA-seq)

We report knockdown of Crebl2 in C2C12 and Hepa1-6 cells to explore its function in metabolism. To understand its role we perform RNS-Seq of control and Crebl2 RNAi cells in both cell lines. C2C12 cells were treated with control (Renilla Luciferase) and Crebl2 siRNAs for three days, RNA was extracted and then sequenced. In parallel some samples for treated with either EtOH or rapamycin for 12 hours to assess Crebl2 role in rapamycin induced transcriptional changes.