Chromosome localization of cohesin oligomers in mid-M arrested yeast cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE157155
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资源简介：
We report the genomic localization of cohesin oligomers in nocodazole arrested yeast cells. Two alleles of SMC3 were expressed in yeast cells, one fused to BirA enzyme and the other tagged with AviTag. Cohesin oligomers were biotinylated and ChIP with streptavidin beads. As control experiments, cohesin localization on chromosome was determined in strains expresses freely diffusable BirA enzyme, where all Smc3 proteins were biotinylated; non-specific ChIP were determined in strains with no BirA. Comparing ChIP results of biotinylated Smc3p-AviTag in strains expressing Smc3p-BirA, NLS-BirA or no BirA.
创建时间：
2021-02-20
