Data from: Larvicidal potential of Pseudomonas mosselii isolated from Aedes (Diptera: Culicidae) egg surfaces against dengue vector mosquitoes and its impact on non-target organisms

The critical impact of mosquito-borne diseases on public health, along
with the failure of current control strategies, highlights the need for
novel mosquito control approaches, especially in tropical countries. This
study explored the larvicidal properties of bacterial isolates from the
egg surfaces of Aedes aegypti L. and Aedes albopictus Skuse (Diptera:
Culicidae), the primary and secondary dengue vector mosquitoes in Sri
Lanka. Among 30 bacterial isolates, a local strain of Pseudomonas mosselii
Quesnel, isolated from the egg surfaces of dengue vectors, demonstrated
strong larvicidal activity against Ae. aegypti. Larvicidal assays using
the bacterial supernatant (OD 1.7) and freeze-dried products were
performed across concentrations ranging from 80 to 2.1 mg/mL. The LC50
values for the supernatant were 55, 54, and 52 mg/mL at 24, 48, and 72 h,
respectively, with corresponding LC90 values of 91, 89, and 86 mg/mL.
Freeze-dried supernatant showed LC50 values of 2.73, 2.64, and 2.63 mg/mL,
while the freeze-dried bacterial culture showed higher toxicity with LC50
values of 2.57, 2.51, and 2.44 mg/mL. Strong larvicidal activity was also
observed against Culex quinquefasciatus Say (Diptera: Culicidae), a
primary vector of filariasis. The freeze-dried products retained their
efficacy for up to two months. Ovicidal and adulticidal assays indicated
no significant effects on mosquito eggs or adults. However, the active
components of bacterial metabolites significantly impacted the development
of Aedes larvae. Toxicity effects were also observed in non-target
organisms, including zooplankton and tadpoles. These findings suggest that
the local Ps. mosselii strain may serve as a potential bio-larvicide
against dengue vectors while underscoring the need for further
environmental assessment.