Quantitative PCR analysis of immune factors in the brains of Aβ-immunized APP/IFN-γ Tg mice.

APP/IFN-γ Tg mice aged 9 months were immunized with Aβ/CFA and co-injected twice with PTX as described in Methods. Untreated APP/IFN-γ Tg mice were used as controls. Mice were analyzed for gene expression in the brain by quantitative PCR as described in Materials and Methods. For each of the genes analyzed, the relative quantity (RQ) was calculated in relation to the level of actin expression. Results are presented as average RQs of the expressed genes in vaccinated mice relative to control mice. P values and SEM were calculated from two immunized mice with substantial T-cell infiltrates in the brain parenchyma and four untreated mice, using the 2-tailed Mann-Whitney test.