wild soybean Raw sequence reads

Soil salinization is a predominant factor restricting plant growth and yield. In this study, common and salt-tolerant wild soybeans were treated with NaCl:Na2SO4 (1:1) at VE stages. Salt tolerance metabolic regulatory modules of the cotyledons were determined by comparing differences in growth, physiology, metabolomics and transcriptomics. We found that salt-tolerant soybeans could maintain relative stability of cotyledon growth parameters and chloroplast microstructure. Additionally, they could sustain the stability of photosynthetic parameters and Ca2+ concentration, increase K+ content, and reduce Na+ accumulation through up-regulating related genes. In salt-tolerant cotyledons, the relative levels of pyruvic acid, shikimic acid, daidzein, loganin, the ascorbic acid precursor inositol and the expression levels of relevant genes, involved in the antioxidant metabolic modules of secondary substances, were significantly increased. In the abscisic acid (ABA) synthesis and signal transduction, the 9-cis-epoxycarotenoid dioxygenase (NCED), PYL, PP2C, SnRK2, and ABF genes showed notable upregulation in the salt-tolerant cotyledons. They also accumulated sucrose, maltose, mannose, malic acid, succinic acid and fumaric acid in the osmoregulatory module and the tricarboxylic acid (TCA) cycle by up-regulating the expression of relevant genes. Therefore, the key salt-tolerance mechanism was to improve the secondary antioxidant metabolism module and promote ABA synthesis and associated signal transduction. Additionally, salt-tolerant cotyledons enhance the osmoregulatory module and TCA cycle. There results will help improve the physiological and biochemical theory of soybean and have important guiding significance for the breeding of new varieties of salt-tolerant soybean plants.