Aldolase A Accelerates Cancer Progression by Modulating mRNA Translation and Protein Biosynthesis via Noncanonical Mechanisms

We provide robust and intensive evidence highlighting a close cooperative mechanistic interaction between ALDOA and IGF2BP1 that fine-tunes mRNA translation and enhances liver cancer progression. Moreover, specifically targeting ALDOA with GalNAc-conjugated siRNA shows promising anti-HCC effect in vivo. our findings uncover a previously unappreciated function of ALDOA in modulating mRNA translation and highlight the potential of targeting ALDOA as a prospective therapeutic strategy in HCC. Overall design: RNA immunoprecipitation followed by sequencing (RIP-seq) was performed using antibody against IGF2BP1 to identify the targeted transcripts enriched by IGF2BP1 in HuH-7 cells. m6A-RNA immunoprecipitation followed by sequencing (MeRIP-seq) was performed to identify the m6A sites across mRNA in HuH-7 cells.