Homing endonuclease I-PpoI-DNA complex:reaction at pH6.0 (K+ MES) with 500 uM Mn2+ for 10s

Homing endonuclease I-PpoI-DNA complex:reaction at pH6.0 (K+ MES) with 500 uM Mn2+ for 10s Descriptor: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3'), GLYCEROL, Intron-encoded endonuclease I-PpoI, ... Authors: Chang, C, Gao, Y. Deposit date: 2024-01-13 Release date: 2024-07-10 Last modified: 2025-01-29 Method: X-RAY DIFFRACTION (1.57 Å) Cite: Observing one-divalent-metal-ion-dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo. Elife, 13, 2024

归巢核酸内切酶（Homing endonuclease）I-PpoI-DNA复合物的反应体系：于pH 6.0（含K+吗啉代乙磺酸（MES））缓冲液中，以500 μM Mn²+为反应试剂孵育10秒。描述项：DNA序列为5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3'、甘油、内含子编码的归巢核酸内切酶I-PpoI……作者：Chang, C、Gao, Y。提交日期：2024-01-13，发布日期：2024-07-10，最后修改日期：2025-01-29。实验方法：X射线衍射（X-RAY DIFFRACTION，分辨率1.57埃）。引用文献：《晶体中观察单二价金属离子依赖与组氨酸介导的His-Me家族I-PpoI核酸酶催化过程》，《eLife》，第13卷，2024年。