Impact of COP1 silencing on hepatoma and hepatocyte transcriptomics. Impact of COP1 silencing on hepatoma and hepatocyte transcriptomics

Transfection experiments aimed at understanding the role of COP1 in hepatocytes and hepatoma models Overall design: HuH-7 cells, HepG2 cells (hepatocyte-derived carcinoma cell line) or a lot of commercial primary hepatocytes (HMCPMS) from ThermoFisher Scientific were transfected for 96 h with 10 nM siRNA targeting either a RFWD2/COP1 or a control (NT1) siRNA. RNA was then harvested and the transcriptome analyzed by transcription array. Each file corresponds to a distinct biologic: the experiment was repeated on 2 -3 distinct occasions.