Induced androgenetic development of rainbow trout - transcriptome analysis of the irradiated eggs

Ionizing radiation (IR) is applied to inactivate nuclear genome in the rainbow trout (Oncorhynchus mykiss) eggs during androgenesis. However, it has been considered that doses of IR used to damage maternal chromosomes may also affect maternal RNA deposited in the cytoplasm during oogenesis. To verify this assumption, quality and quantity of RNA extracted from the irradiated rainbow trout eggs were studied using standard molecular techniques and RNA-Seq approach.