Hans Ris (2011) CIL:10722, Notophthalmus viridescens, oocyte. CIL. Dataset

Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo examination of the pair provides an oblique 3D view.

梅奥蒂新鳟卵母细胞核在30 mM KCl/NaCl溶液中分散，经2%甲醛固定后离心至电子显微镜载网，用铀酸乙酸染色，并进行临界点干燥。载网在Madison 1MeV透射电子显微镜下以10 KX倍率进行观察。该显微照片展示了环状梳状染色体上的转录过程，拍摄时倾斜角度为45度。该图像与同一样本区域以55度倾斜角度拍摄的图像进行分组。对该对图像进行立体观察，可获得斜向的三维视图。