Nicking Mutagenesis Raw Sequening Reads
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下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP083894
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资源简介:
The purpose of this study was to demonstrate the efficacy of Nicking Mutagenesis, a new comprehensive single-site saturation mutagenesis (CSM) method, on multiple gene targets. Briefly, genes of interest were ordered synthetically and cloned into an E coli expression plasmid. Specified regions of the genes were targeted to generate CSM libraries.
创建时间:
2016-09-27



