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Nicking Mutagenesis Raw Sequening Reads

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NIAID Data Ecosystem2026-03-09 收录
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The purpose of this study was to demonstrate the efficacy of Nicking Mutagenesis, a new comprehensive single-site saturation mutagenesis (CSM) method, on multiple gene targets. Briefly, genes of interest were ordered synthetically and cloned into an E coli expression plasmid. Specified regions of the genes were targeted to generate CSM libraries.

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2016-09-27
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