Biological variation observed in baseline Top1 levels in xenograft models and patient samples.

Abbreviations: PBMCs, peripheral blood mononuclear cells; SD, standard deviation.
a. Xenograft data include Top1 levels measured in untreated and vehicle-treated (water) A375 and HCT 116 xenograft bearing mice and vehicle-treated (water) Colo829 and SK-MEL-28 mice from all experiments.
b. Top1 levels measured in tumor biopsies from patients at baseline. Top1 levels expressed as an average of all measurements above the LLQ; each patient had Top1 levels above the LLQ for at least 2 of 3 protein loads.
c. Top1 levels measured in PBMC samples from patients at baseline. Top1 levels expressed as an average of all measurements above the LLQ. In one patient, the Top1 levels were below the LLQ, in this case the LLQ was used as the Top1 measurement.