The number of neurons expresses GFRα1-3, TRPV1 and ATF3 mRNA out of 20 IB4-labeled neurons in DRG after axotomy.

In naïve (non-axotomized) mice, all IB4-back-labeled neurons expressed GFRα2 mRNA. However, GFRα2 mRNA expression was rarely detected 1 and 6 d post-axotomy. The number of IB4-back-labeled neurons expressing GFRα1, GFRα3 and TRPV1 mRNA increased significantly 6 d post-axotomy. ATF3 mRNA expression was detected in all IB4-back-labeled neurons 1 d after nerve transection and remained at this level for at least 6 d. (n = 3, *p