Chromatin immunoprecipitation and sequencing of pPLT2::PLT2-YFP in the root of plt1plt2 double mutant

Organ formation in animals and plants relies on precise control of cell state transitions to turn stem cell daughters into fully differentiated cells. In plants, cells cannot rearrange due to shared cell walls. Thus, differentiation progression and the accompanying cell expansion must be tightly coordinated. PLETHORA (PLT) transcription factor gradients were shown to guide the progression of cell differentiation at different positions in the Arabidopsis root. While well-described transcription factor gradients in animals specify distinct cell fates within an essentially static context, the PLT gradient is unique in its ability to control cell differentiation in a growing organ during continuous production and expansion of cells. To understand the output of these gradients we studied the gene set transcriptionally controlled by PLTs. Our work reveals how the PLT gradient regulates cell state by region-specific induction of cell proliferation genes and repression of differentiation. Moreover, PLT targets include major patterning genes and autoregulatory feedback components, reinforcing their role as master regulators of organ development.

动植物的器官发生依赖于对细胞状态转变的精准调控，从而将干细胞子代转化为完全分化的细胞。由于植物细胞拥有共享的细胞壁，无法发生重排，因此细胞分化进程与伴随的细胞扩张必须被紧密协同调控。PLETHORA（PLT）转录因子梯度已被证实，可指导拟南芥根系不同位置的细胞分化进程。尽管已被充分研究的动物转录因子梯度可在基本静态的环境中界定不同的细胞命运，但PLT梯度的独特之处在于，它能在器官持续产生并扩张细胞的过程中，调控生长器官内的细胞分化。为解析这类梯度的调控效应，我们研究了受PLT转录调控的基因集合。本研究揭示了PLT梯度如何通过区域特异性诱导细胞增殖基因、抑制分化进程，来调控细胞状态。此外，PLT的靶基因包含主要的模式建成基因与自调控反馈元件，进一步证实了其作为器官发育主控调控因子的核心地位。

搜集汇总

背景与挑战

背景概述

该数据集通过ChIP-Seq技术研究了拟南芥根中PLETHORA（PLT）转录因子梯度对细胞分化的调控机制，包含3个样本（输入DNA和PLT2_IP），总数据量6.67 GB。研究发现PLT梯度通过区域特异性诱导细胞增殖基因和抑制分化基因来调控细胞状态，并涉及主要模式基因和自调节反馈组件，揭示了其在器官发育中的核心调控作用。