Genetic reporter for live tracing fluid flow forces during cell fate segregation in mouse blastocyst development. Genetic reporter for live tracing fluid flow forces during cell fate segregation in mouse blastocyst development

We used a robut low input cell and high throughput in-situ ChIP method to emphasize regulation mechanism among E3.5 klf2, E3.5 klf4 and E12.5 EC klf2. Overall design: We fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing