Next Generation Sequencing of CD8+ and CD4+ cells isolated from angiotensin-2 treated mice

RNA-sequencing of CD8 lymphocytes isolated from pre-hypertensive mice revealed upregulation of gene pathways involved in MAPK cascade, positive regulation of intracellular calcium fluxes, response to IFN-g and other external stimuli, and lymphocyte chemotaxis, as compared to CD4 T cells. Taken together these results indicate that hypertensive stimuli program CD8 T cells toward a phenotype with pro-migratory properties that might account for their ability to enhance myogenic tone of resistance arteries Overall design: RNA sequencing of FACS-sorted CD4+ and CD8+ cells from Angiotensin-2 treated mice