The performance of long-range PE sequencing libraries.

*Proper, properly mapped long-range PE reads; Small, small insert PE reads contamination; and Chimeric, chimerical reads.
Duplicates were defined as PE reads that have precisely the same sequences on both ends; the duplication rate was calculated by one minus the percent of the non-redundant PE reads in total reads. For each library, the normal long-range PE reads were defined as the uniquely mapped read pairs that have correct orientation relationships and insert-sizes lying within ±3SD; small insert read contamination represent the uniquely mapped read pairs that have the orientation relationships and insert sizes of a standard ∼500-bp spanning library; and chimeric reads represent the remaining reads excluding the above two classes of read-pair.