scRNA-seq of human engineered heart tissue for analyzing human myocardial fibrosis

Myocardial infarction models developed to understand fibrosis and promote myocardial regeneration towards ischemic heart disease (IHD) is challenged due to the low-throughput nature of in vivo models and the lack of humanized cardiac scarring in the in vitro models. We generated a novel 4D cardiac scarring model (4DCSM) mimicking the IHD in vitro based on the human engineered heart tissue(hEHT). To identify potential signaling dominating the fibrotic process, we characterized transcriptomics of 4DCSM at different scarring stages by single-cell RNA sequencing. Taken together, our dataset is an informative resource to reveal the critical cell-types fate transformation and in fibrotic process and provides potential comparisons with public clinical data cohorts or other MI fibrosis model data. The sample was human cardiac scarring model based on based on the hEHT derived from human pluripotent stem cells, we collected three biological replicates of the hEHT after 3day cultivation, cryoinjured hEHT after 2day and 4day cultivation, and non-cryoinjured hEHT after 2day and 4day cultivation.