Intensity of 0ΔNLS antiserum IgG binding of HSV-2 Proteins by MW: Peak Analysis.

a Numbers correspond to protein bands denoted by red diamonds in Figs. 1 and 2. b Apparent molecular weight of proteins bound by IgG in mouse 0ΔNLS antiserum. c Mean ± sem intensity of IgG binding to each of 9 HSV-2 proteins, as calculated from the percentage of “peak signal associated with each of the 9 bands in the HSV-2 lane” relative to the “summation of peak signals in all 9 protein positions on the same blot.” The mean ± sem of these values were calculated from n = 5 blots incubated with the serum of 1 of 5 mice immunized with HSV-2 0ΔNLS (S1 Fig.). Intensity of 0ΔNLS antiserum IgG binding of HSV-2 Proteins by MW: Peak Analysis.