Gene expression change of M1 and BY-P41K-RPB7 in YPAD (OD600 reached 1) and after 12h Very High Gravity (VHG) fermentation. Saccharomyces cerevisiae

To further study the transcription profile of mutant M1 and its control (BY-P41K-RPB7), we have employed whole genome microarray expression profiling as a discovery platform to identify differentially expressed genes in YPAD medium under OD600 reached 1 and after 12 VHG fermentation. Overall design: Total cellular RNAs were extracted from both the mutant and the control using RNeasy® Mini Kit and RNase-Free DNase Set (Qiagen,Hilden, Germany) under the following two conditions: i) when cells reached early exponential phase (OD600 ~1.0) in YPAD; ii) after 12h VHG fermentation. RNA quality and integrity was verified by gel electrophoresis, as well as by measuring 260/230 ratios with a NanoDrop 1000 spectrophotometer (Thermo Scientific, MA, USA). Two biological replicates of each sample were sent to Genomax Technologies (Singapore) for DNA microarray assay using Yeast (V2) Gene Expression Microarray, 8×15K Microarrays (Agilent technologies, USA). The obtained data was analyzed by Agilent Genespring GX software and the p-values were calculated by unpaired Student t-test.