Single nucleotide resolution maps of Illumina sRNA-seq reads representing viral siRNAs from BBTV-infected Cavendish banana plants with or without DRC alphasatellite.
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For each of the two conditions, i.e. without (BBTV) and with (BBTVa) alphasatellite, Illumina 20–25 nt reads of the three biological replicates (leaf tissues of three plants) were combined and mapped simultaneously onto the reference sequences of (C, M, N, R, S, U3) and DRC-2016 alphasatellite (alpha), extended at the 3-end by 33 nts from the 5-terminal sequence to allow for mapping sRNAs derived from the circular viral genome. BAM files with mapped viral reads were analyzed by MISIS-2 [80] yielding the count tables of forward and reverse reads of each size-class, starting or ending at respective positions of the reference sequences. (XLSX)
创建时间:
2022-04-12



