Umbilical cord as a long-term source of activatable MSC for immunomodulation

To facilitate umbilical cord explant culture as a long-term source for mesenchymal stromal cells, we compared the transcriptome of mesenchymal stromal cell explant cultures (MSC-EM) initiated 14 days (early) post preparation, 2 months later (late) and with INF-g and TNF-a (25 ng/mL) activated samples We compared the early and late induced mesenchymal stromal cell explant (MSC-EM) cultures from human umbilical cord pieces from several donors versus activated samples. MSC-EMs 1 are early. MSC-EMs 6 and 7 are late.