A longitudinal RNA sequencing dataset in primary human fibroblasts with mitochondrial genetic, metabolic, and endocrine perturbations

The current RNA sequencing dataset (n=345) provides a transcriptional map of cultured primary human fibroblasts (in vitro) aged through the cellular lifespan. The dataset was generated from paired-end (150bp) Illumina HiSeq sequencing, with an average coverage of 38.3million reads per sample. These data contain gene expression trajectories collected at high temporal frequency (every 11 days, ~7timepoints per donor) across the replicative lifespan of primary human fibroblast cell lines from n=4 healthy male and female donors, and n=3 donors with lifespan-altering mitochondrial disease caused by mutations in the SURF1 gene. The dataset can be integrated with other measures collected in parallel along the lifespan, including cytological (cell size, morphology), bioenergetic (energy expenditure, derived ATP synthesis rates from Seahorse), epigenetic (DNA methylation, GSE179847), secreted proteins, telomere length, and whole-genome sequencing (WGS) data. This dataset also includes experimental manipulations with treatments targeting oxidative phosphorylation (OXPHOS) and glycolysis, and glucocorticoid signaling, providing an opportunity to examine the influence of stress and bioenergetics on human aging biology. All processed data can be accessed and browsed at our webtool: https://columbia-picard.shinyapps.io/shinyapp-Lifespan_Study/ Overall design: Primary human fibroblasts are cultured and passaged over ~250 days of replicative lifespan in various treatment conditions.