Liquid culture fluorescence data of the GFP insertion constructs expressing four test proteins with progressively decreasing solubility at 37��C.

aProtein #1 sulfite reductase (dissimilatory subunit); protein #2 (translation initiation factor), protein #3 (3-hexulose 6-phosphate synthase), and protein #4 (polysulfide reductase subunit).bFraction of non-fusion protein soluble expressed in E. coli at 37��C, as determined by SDS-PAGE.cScaffolding topology of GFP folding reporter.dType of GFP domain used in reporter, SF = superfolder GFP, FR = folding reporter GFP.eThe measured fluorescence (488 nm excitation, 520 nm emission) normalized by dividing by the optical density at 600 nm.