A bioengineered tridimensional substrate designed for mesenchymal stem cells culture: whole transcriptomic analysis

In this work we aimed to evaluate how the three-dimensionality of an engineered scaffold, concieved to emulate the stem cell niche, can influence the whole gene expression profile of mesenchymal stem cells. We performed a differential expression analysis, comparing the transcriptome of MSCs grown in 3D Nichoids with that of MSCs grown on 2D Nichoids, a structure with the same grid geometry, except for the overall tickness which allows a spreading expansion of cells. We found a total of 603 differentially expressed genes that were used also for functional enrichment analysis. Total RNA extracted from rBMSCs cultured on 3D Nichoid (n=3) and 2D Nichoid (n=3) after 4 days of culture