Antimicrobial and hemolytic activities and prokaryotic selectivity (therapeutic index) of designed HDAMPs.

aMinimal inhibitory concentration of peptides in µg/mL; values in parentheses are in µM.bThe geometric mean (GM) of the MIC values against four bacterial strains in µg/mL; values in parentheses are in µM. When no detectable antimicrobial activity was observed at the maximum concentration (64 µg/mL), the value of twice the maximum concentration (128 µg/mL) was used to calculate the therapeutic index.cHC10 is the peptide concentration that induces 10% hemolysis against human erythrocytes; in µg/mL; values in parentheses are in µM. When no detectable hemolytic activity was observed at the maximum concentration (256 µg/mL), the value of twice the maximum concentration (512 µg/mL) was used to calculate the therapeutic index.dThe therapeutic index was calculated as the ratio of the HC10 to the GM. Larger values of TI correspond to greater prokaryotic selectivity.