Identification of pathological pathways centered on circRNA dysregulation associated with irreversible progression of Alzheimer’s disease

To experimentally validate circGigyf2-CPSF6 interaction, we performed circGigyf2 pulldown as illustrated in Fig. 6G. A robust and specific enrichment of CPSF6 was found in circGigyf2 pulldown (Fig. 7C). In contrast, Quaking (QKI) and TAR DNA-binding protein 43 (TDP-43), two RBPs that are predicted to have negligible association with circGigyf2 body, were not detected in circGigyf2 pulldown (Fig. 7C). The non-RBP Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) was also undetected (Fig. 7C). These data demonstrated specific interactions between circGigyf2 and CPSF6.